Scientist taking part in protein engineering and directed advancement experiments screen alternative libraries to determine proteins with desirable features. To construct these libraries, researchers frequently present random DNA mutations by error-prone PCR (epPCR), however this technique experiences amplification predisposition, incomplete alternative representation, and premature stop codons. Site Saturation Variant Libraries (SSVLs) are synthetic, designed alternatives to libraries created by random epPCR. Since each mutant is directly synthesized, SSVLs offer near-complete variant representation, offering researchers with a complete collection of possible proteins.Download this application note from Twist Bioscience to see how an SSVL outshines a random epPCR library in a protein engineering experiment.Sponsored by