December 23, 2024

Single-Cell Spatial Transcriptomics with Cleavable Fluorescent Probes  

This webinar will be hosted readily available and live on-demandTuesday, November 22, 2022 2:30 PM Eastern Time Scientists use in situ hybridization (ISH) to expose the area of particular nucleic acid sequences within cells, which is an important step for understanding gene company, regulation, and function. In situ hybridization can maintain spatial information, which avoids the masking of cellular heterogeneity that comes with bulk analysis. In this Technique Talk, Jia Guo will explore the principles of ISH and the development and usage of unique in situ hybridization technology to investigate complex biological systems.Learning ObjectivesHow multiplexed fluorescent ISH worksMild yet efficient techniques for eliminating signalWays to magnify signalDetecting brief RNA types in formalin-fixed paraffin-embedded (FFPE) tissuesJia Guo, PhDAssociate ProfessorBiodesign Institute and School of Molecular SciencesArizona State University