With this new technology in hand, Takahashi might start his research study on the inheritance of epigenetic changes.An unedited mouse (left) compared to the original methylation modified mouse (P0) and his grandchild (F2) carrying the exact same epigenetic mutation.Yuta TakahashiUsing their DNA methylation modifying technology, the researchers silenced 2 genes associated with metabolic process (of interest due to their connection to illness such as diabetes and weight problems) in mouse stem cells. As they d hoped, the mice with epigenetically customized cells showed phenotypic modifications; they became more obese than control mice and had greater cholesterol levels.They then bred a male modified mouse to a female mouse from an unrelated pressure. After purifying and evaluating the methylation patterns of the samples, they found that all offspring mice throughout the four generations who inherited the modified allele displayed the prevented methylation pattern, no matter if a male or female edited mouse was the parent.
With this new innovation in hand, Takahashi might start his research study on the inheritance of epigenetic changes.An unedited mouse (left) compared to the initial methylation edited mouse (P0) and his grandchild (F2) carrying the same epigenetic mutation.Yuta TakahashiUsing their DNA methylation modifying technology, the scientists silenced two genes associated with metabolic process (of interest due to their connection to diseases such as diabetes and weight problems) in mouse stem cells. They then injected those stem cells into mouse embryos, placed the embryos into surrogate moms and observed the mice from birth to 10 months of age. As they d hoped, the mice with epigenetically customized cells displayed phenotypic changes; they became more obese than control mice and had higher cholesterol levels.They then bred a male modified mouse to a female mouse from an unrelated pressure. They then selected one male and one female offspring mouse that had acquired the customized allele and bred each of them to mice from the same unrelated stress, continuing this procedure for 4 generations. After cleansing and analyzing the methylation patterns of the samples, they discovered that all offspring mice throughout the four generations who acquired the modified allele displayed the prevented methylation pattern, no matter if a female or male edited mouse was the moms and dad.
