In this Technique Talk, R. Hal Scofield will supply tips and ideas for acquiring the best possible information utilizing current approaches and preventing common pitfalls.Learning ObjectivesThe standard requirements for generating tidy and reproducible qualitative and quantitative western blot dataHow to deal with low abundance proteinsKeys to identifying between 2 proteins with comparable molecular weightsSteps for minimizing non-specific signalR. Hal Scofield, MDMember, Arthritis and Clinical Immunology ProgramOklahoma Medical Research FoundationProfessor, Department of MedicineUniversity of Oklahoma Health Sciences CenterAssociate Chief of Staff for Research and Staff PhysicianOklahoma City United States Department of Veterans Affairs Medical CenterSponsored by