The lower temperature level in RPA increases the risk of nonspecific amplification, so Merkx sought a sensitive option.”The appeal of bioluminescence is its simpleness,” said Merkx. The team evaluated this platform on COVID-19 samples and detected SARS-CoV-2 RNA within 30 minutes.
The lower temperature in RPA increases the danger of nonspecific amplification, so Merkx looked for a delicate service.”The charm of bioluminescence is its simplicity,” stated Merkx. The team split luciferase, an enzyme that produces bioluminescence, between 2 dCas9 proteins. The group evaluated this platform on COVID-19 samples and found SARS-CoV-2 RNA within 30 minutes.
