Due to the fact that of its imaging capabilities, the BD FACSDiscover ™ S8 Cell Sorter enables scientists to distinguish in between cells in various cell cycle phases.Flow cytometry is a single-cell analytical technique that uses fluorophore-labeled cell structures or biomarkers to separate between cell populations. Through this method, scientists detect the fluorescence intensity of several cellular biomarkers, in addition to the cells relative size and granularity. However, scientists can just utilize a restricted variety of fluorophores concurrently, as it can be difficult to compare fluorophores with overlapping emission spectra.Flow Cytometry VariationsTo increase its flexibility, researchers developed several circulation cytometry variations, including fluorescence-activated cell sorting (FACS), imaging circulation cytometry, and spectral flow cytometry. FACS is a technique that utilizes conventional circulation cytometry to determine cell populations with specific functions and enables the user to sort the chosen group into a collection vessel for additional propagation or analysis.1 This method enables researchers to separate a selected cell type from a heterogeneous sample, activated cells from non-activated cells, or cells revealing a specific protein from non-expressing cells.Imaging circulation cytometry is a technique that combines conventional flow cytometry with microscopy and permits the user to analyze a fluorophore-labeled cells fluorescence and morphology. In this method, the instrument lights up cells through filtered white light, producing brightfield conditions. Video cameras then catch both brightfield and fluorescent images. Like microscopy, imaging circulation cytometers permit researchers to determine a fluorophores extracellular and intracellular distribution. This technique also makes sure that cellular debris and cell clusters are not included in the analysis.2 Furthermore, imaging circulation cytometry is a high-throughput approach, so researchers analyze cells faster than microscopy-based imaging. Unlike conventional circulation cytometry, the brightfield images likewise let scientists figure out exact cell sizes instead of relative sizes.2 Spectral flow cytometry is a sophisticated variation of standard flow cytometry. In this approach, the instrument examines a fluorophores whole emission spectrum, instead of only a little portion, like traditional circulation cytometers.3 This makes it possible for spectral circulation cytometers to discriminate in between fluorophores with comparable emission spectra, which increases the number of fluorophores that scientists can use concurrently to separate cell populations. While scientists have actually previously integrated a few of these approaches, no instrument made use of all their benefits up until recently. An Innovative and Efficient SolutionFor labs dealing with biohazardous samples, the BD FACSDiscover ™ S8 Cell Sorter can be installed within a custom-designed biosafety cabinet.BD BiosciencesThe BD FACSDiscover ™ Cell Sorter with BD CellView ™ Image Technology and SpectralFX ™ Technology from BD Biosciences is the first instrument that integrates FACS, imaging circulation cytometry, and spectral flow cytometry. The instrument incorporates morphological details stemmed from real-time imaging with fluorophore-based information gotten from flow cytometry, which enables researchers to recognize and sort cell subgroups.The BD FACSDiscover ™ S8 Cell Sorter utilizes its 78 fluorescent detectors to take a look at a fluorophores full spectrum, thereby taking full advantage of the number of fluorophores that scientists can utilize simultaneously. Furthermore, the instrument permits high-speed sample sorting, where researchers can separate six selected cell populations into 5mL collection tubes. The real-time images permit the user to include spatial information about the fluorophore and cell into the decision-making process required for sorting. Through BD CellView ™ Image Technology, the BD FACSDiscover ™ S8 Cell Sorter utilizes its electronic and optical components to image cells without a video camera, causing faster imaging. Additionally, the instrument produces multicolor brightfield-like images and fluorescent images. Common scatter and fluorescence analysis, the BD FACSDiscover ™ S8 Cell Sorter makes it possible for researchers to evaluate 12 cell image features, consisting of correlation, eccentricity, delta center of mass, and diffusivity.Due to its flexibility and optional placement within custom-made biosafety cabinets, scientists from fields such as cell biology, immunology, oncology, and microbiology can use the BD FACSDiscover ™ S8 Cell Sorter to study a number of subjects, including phagocytosis, growth cell killing, and the cell cycle. In general, the BD FACSDiscover ™ Cell Sorter with BD CellView ™ Image Technology and SpectralFX ™ Technology includes the advantages of multiple flow cytometry variations to produce a versatile and ingenious instrument.ReferencesMcKinnon KM. Circulation cytometry: An overview. Curr Protoc Immunol. 2018; 120:5.1.1 -5.1.11. Han Y, et al. Review: Imaging innovations for circulation cytometry. Lab Chip. 2016; 16( 24 ):4639 -4647. Robinson JP. Flow cytometry: past and future. BioTechniques. 2022; 72( 4 ):159 -169..
Since of its imaging abilities, the BD FACSDiscover ™ S8 Cell Sorter allows scientists to distinguish between cells in various cell cycle phases.Flow cytometry is a single-cell analytical method that uses fluorophore-labeled cell structures or biomarkers to differentiate between cell populations. FACS is a technique that utilizes conventional circulation cytometry to determine cell populations with particular features and allows the user to sort the selected group into a collection vessel for additional propagation or analysis.1 This method allows scientists to isolate a chosen cell type from a heterogeneous sample, triggered cells from non-activated cells, or cells revealing a particular protein from non-expressing cells.Imaging flow cytometry is a technique that integrates standard flow cytometry with microscopy and allows the user to examine a fluorophore-labeled cells fluorescence and morphology. An Innovative and Efficient SolutionFor laboratories working with biohazardous samples, the BD FACSDiscover ™ S8 Cell Sorter can be set up within a custom-designed biosafety cabinet.BD BiosciencesThe BD FACSDiscover ™ Cell Sorter with BD CellView ™ Image Technology and SpectralFX ™ Technology from BD Biosciences is the very first instrument that integrates FACS, imaging circulation cytometry, and spectral circulation cytometry. The instrument integrates morphological information obtained from real-time imaging with fluorophore-based data obtained from circulation cytometry, which allows researchers to sort and identify cell subgroups.The BD FACSDiscover ™ S8 Cell Sorter utilizes its 78 fluorescent detectors to take a look at a fluorophores complete spectrum, thus optimizing the number of fluorophores that scientists can use concurrently. Typical scatter and fluorescence analysis, the BD FACSDiscover ™ S8 Cell Sorter allows scientists to assess 12 cell image functions, including connection, eccentricity, delta center of mass, and diffusivity.Due to its flexibility and optional positioning within custom-made biosafety cabinets, researchers from fields such as cell biology, immunology, oncology, and microbiology can utilize the BD FACSDiscover ™ S8 Cell Sorter to study a number of subjects, consisting of phagocytosis, growth cell killing, and the cell cycle.