Fifteen C57BL/6 male mice aged 12– 18 months with old-age-induced obesity were divided into 3 groups. Group 1 was offered a regular diet supplemented with non-recombinant E. coli JM109 cells orally by gavage two times daily; Group 2 was offered a regular diet supplemented with recombinant E. coli JM109-rMETase cells by gavage twice daily; and Group 3 was provided a methionine-deficient diet plan without treatment.
The administration of E. coli JM109-rMETase or a methionine-deficient diet plan lowered the blood methionine level and reversed old-age-induced obesity with significant weight reduction by 14 days. There was a negative connection in between methionine levels and unfavorable body weight change. The degree of effectiveness was greater in the methionine-deficient diet group than in the E. coli JM109-rMETase group, the present findings suggested that oral administration of E. coli JM109-rMETase, as well as a methionine-deficient diet, are effective in reversing old-age-induced weight problems.
” In conclusion, the present research study provides evidence that restricting methionine by either a low-methionine diet plan or E. coli JM109-rMETase has medical capacity to treat old-age-induced weight problems.”
Reference: “Old-age-induced weight problems reversed by a methionine-deficient diet plan or oral administration of recombinant methioninase-producing Escherichia coli in C57BL/6 mice” by Yutaro Kubota, Qinghong Han, Jose Reynoso, Yusuke Aoki, Noriyuki Masaki, Koya Obara, Kazuyuki Hamada, Michael Bouvet, Takuya Tsunoda and Robert M. Hoffman, 9 June 2023, Aging.DOI: 10.18632/ aging.204783.
Mouse body weight increases with age, however obesity in older mice was reversed with a methionine-deficient diet plan or treatment with methionine-restricting E. coli JM109-rMETase. Both methods substantially decreased methionine levels, with the diet plan being more effective, and did not decrease lean muscle mass. Humans find it tough to restrict methionine by diet alone, oral methioninase and crafted E. coli have actually shown pledge in decreasing blood methionine levels, suggesting possible clinical applications for reversing old-age-induced obesity.
” This is the very first report that revealed the efficacy of methionine restriction to reverse old-age-induced weight problems.”
Methionine constraint impacts lipid metabolism and can prevent weight problems in mice. A current study published in the journal Aging found that restricting the amino acid methionine impacts fat metabolic process and might help prevent weight problems.
The team then assessed the effectiveness of oral administration of recombinant-methioninase (rMETase)- producing E. coli (E. coli JM109-rMETase) or a methionine-deficient diet plan to reverse old-age-induced weight problems in C57BL/6 mice.
” In the present research study, we checked a low-methionine diet plan to reverse old-age-induced obesity. E. coli JM109-rMETase was likewise evaluated in the present research study to reverse old-age-induced weight problems.”
Human beings discover it tough to limit methionine by diet plan alone, oral methioninase and engineered E. coli have actually revealed guarantee in decreasing blood methionine levels, recommending possible scientific applications for reversing old-age-induced weight problems.
Group 1 was provided a normal diet plan supplemented with non-recombinant E. coli JM109 cells orally by gavage twice daily; Group 2 was offered a regular diet supplemented with recombinant E. coli JM109-rMETase cells by gavage two times daily; and Group 3 was offered a methionine-deficient diet plan without treatment.
The administration of E. coli JM109-rMETase or a methionine-deficient diet plan lowered the blood methionine level and reversed old-age-induced obesity with significant weight loss by 14 days. The degree of efficacy was greater in the methionine-deficient diet plan group than in the E. coli JM109-rMETase group, the present findings recommended that oral administration of E. coli JM109-rMETase, as well as a methionine-deficient diet, are reliable in reversing old-age-induced weight problems.
