In addition, it has long been known that beer aroma modifications throughout the brewing process and storage because, among other things, part of the (R)- linalool primary in hops is transformed to (S)- linalool.
Formerly, based upon a 1999 paper, scientists presumed that the smell limit concentration of (R)- linalool has to do with an aspect of 80 lower than that of (S)- linalool. In other words, they assumed that (R)- linalool has a much stronger influence on beer aroma than its mirror-image equivalent. Reputable data on the smell thresholds of both compounds were lacking.
Preparative method optimized
To close this knowledge space and enable more exact forecasts of modifications in beer fragrance, the group led by developing and drink technologist Klaas Reglitz and food chemist Martin Steinhaus from the Leibniz Institute initially enhanced a preparative technique. In close cooperation with the Research Center Weihenstephan for Brewing and Food Quality, the researchers therefore was successful for the very first time in isolating enantiomerically pure (S)- linalool.
Image of typical hop (Humulus lupulus) on white background. The typical hop is mainly utilized for beer production. For developing, the cones of the female hop plants are utilized. Credit: Gisela Olias/ Leibniz-LSB@TUM
Having the pure substance available in sufficient amounts was an indispensable requirement for identifying the specific odor threshold concentrations of the two odorant variants in water and unhopped beer using an experienced sensory panel. This was necessary due to the fact that only (R)- linalool is commercially readily available as a pure compound.
As the team showed, the limits of (R)- and (S)- linalool in water were 0.82 and 8.3 micrograms per kg, respectively. In unhopped beer, the team identified limits of 6.5 micrograms per kg for (R)- linalool and 53 micrograms per kilogram for (S)- linalool.
Impact of (R)- linalool overstated
” Our results thus confirm the formerly postulated greater smell potency of (R)- linalool. They likewise refute the previous assumption that the odor threshold concentrations of the 2 enantiomers differ incredibly. Rather, the study reveals that the distinction is only about 8 to significantly,” states Martin Steinhaus, head of Section I and the Food Metabolome Chemistry Research Group at the Leibniz Institute.
First author Klaas Reglitz adds, “The conversion of (R)- to (S)- linalool hence does not have as fantastic an influence on beer fragrance as has long been presumed. Thanks to our study, we now better understand how and why the fragrance modifications throughout storage.”
Referral: “Enantiospecific decision of the odour limit concentrations of (R)- and (S)- linalool in water and beer” by K. Reglitz, J. Stein, J. Ackermann, V. Heigl, L. Brass, F. Ampenberger, M. Zarnkow and M. Steinhaus, July 2023, Brewing Science.DOI: 0.23763/ BrSc23-07reglitz.
By Leibniz Institute for Food Systems Biology at the Technical University of Munich
November 28, 2023
A new study difficulties existing assumptions about linalool, an essential part affecting beers aroma. The research study reveals that the smell limit difference between linalools enantiomers is significantly less than previously believed, offering brand-new insights into the development of beers scent throughout the developing procedure and storage.
Hop-derived components not just enhance the shelf-life and include a bitter taste to beer, but they likewise play a crucial role in affecting its scent. A crucial hop odorant is linalool, identified by its flower and citrusy fragrance.
Under the leadership of the Leibniz Institute for Food Systems Biology at the Technical University of Munich, a group of scientists has actually now negated a roughly 20-year-old assumption about this odorant. The brand-new research study adds to a much better understanding of modifications in beer bouquets throughout the developing process and beer aging.
Two molecular versions of the odorant linalool are found in hops and beer: the enantiomers (R)- and (S)- linalool. Both particles consist of the very same number and kind of atoms, and reveal the exact same connection. They have different spatial structures and differ like an image from its mirror image. This “small” however nonetheless vital difference is likewise reflected in the various smell strengths of the particles.
2 molecular versions of the odorant linalool are found in hops and beer: the enantiomers (R)- and (S)- linalool. Formerly, based on a 1999 paper, scientists assumed that the odor limit concentration of (R)- linalool is about an element of 80 lower than that of (S)- linalool. Put just, they assumed that (R)- linalool has a much more powerful impact on beer scent than its mirror-image counterpart. The common hop is mainly utilized for beer production.” Our outcomes therefore verify the formerly postulated higher odor strength of (R)- linalool.
