PHAGE DISPLAY LIBRARY GENERATION DESIGNED BY ERIN LEMIEUXScientists insert variable DNA sequences coding for their proteins of interest into plasmids that carry a phage coat protein gene, an antibiotic resistance gene, and a packaging signal. They transform the plasmids into a bacterial vector and contaminate it with a helper phage that provides the other essential viral proteins. AFFINITY PURIFICATION DESIGNED BY ERIN LEMIEUXIn the next step, scientists separate the phages revealing surface proteins using a surface area ligand binding assay.
