An innovative technique established at Karolinska Institutet and affiliated organizations enables accurate identification and localization of immune cell receptors, transforming the understanding and treatment of diseases like cancer and autoimmune conditions. Credit: SciTechDaily.comA revolutionary strategy for pinpointing immune cell receptors in tissues advances illness research and treatment development.A brand-new technique, established at Karolinska Institutet, KTH Royal Institute of Technology, and SciLifeLab, can identify special immune cell receptors and their place in tissue, a study published in the journal Science Reports. The scientists forecast that the approach will enhance the ability to determine which immune cells add to disease procedures and open chances to establish unique treatments for numerous diseases.Immune cells such as T and B cells are central to the bodys defense versus both infections and growths. Both types of immune cells reveal special receptors that specifically recognize various parts of foreign and undesirable aspects, such as germs, infections, and tumors. Each immune cell and its progeny has its own particular receptors, and in each human body there are billions of different immune cells with unique receptors.Camilla Engblom. Credit: Johannes FrandsenResearchers at Karolinska Institutet, KTH Royal Institute of Technology, and SciLifeLab have actually now developed an approach that is able to both recognize the different B and T cell receptors and reveal their area in human tissue.Many Areas of Application”Since triggered immune cells are frequently discovered close to the targets that they assault, we wish to have the ability to map the cells that are undoubtedly closest to a tumor or infection,” states Camilla Engblom, assistant teacher at the Department of Medicine (Solna), Karolinska Institutet and one of the studys three lead authors in addition to Kim Thrane, KTH/SciLifeLab, and Qirong Lin, Karolinska Institutet.”It hasnt been possible to recognize both B and T cell receptors in their microenvironments using previous techniques.”According to Dr. Engblom, there is a vast array of locations in which the brand-new technique can be put to scientific use in the future.Jeff Mold. Credit: Sarantis Giatrellis”In cancer, the approach can identify T cells that potentially attack the tumor,” she states. “They could then be used as cell treatment against cancer. We can likewise identify distinct receptors on the B cells that are launched as antibodies in particular areas of the growth. These antibodies can be produced in the lab with relative ease and ultimately provide rise to unique treatments.”Another field is autoimmune illness, where the immune system attacks healthy tissue. The brand-new method could be utilized to identify the immune cells that do this and increase the possibilities of finding precisely what it is they attack.”An Important Step ForwardJeff Mold, among the primary detectives of the research study and researcher at the Department of Cell and Molecular Biology at Karolinska Institutet, sees the new approach as an important action forward.”Identifying these unique immune receptors is like trying to find a needle in a haystack, particularly when it concerns autoimmune illness,” he says. “With most current techniques, you damage the tissue, which implies not just that you get different immune cells blended up, but also that some cells die while doing so. With this technique, we maintain the cells where they are and we can see cells that would otherwise have been lost.”Dr. Mold believes that the capability to determine B cells is arguably the primary benefit of this new technique.”T cells have actually been a popular research target, while the B cells have been a little neglected, particularly in cancer,” he states. “But now we can track how B cells develop and broaden direct in tissue.”Facts: Spatial Transcriptomics of Immune CellsThe method of spatial transcriptomics was developed in 2016 by professors Jonas Frisén at Karolinska Institutet and Joakim Lundeberg at KTH Royal Institute of Technology, who are co-authors of this study. It was called “Method of the Year 2020” by the journal Nature Methods.The brand-new approach is an upgrade of the initial technique that now makes it possible for researchers to map the immune cells receptors and their specific location in tissue, which was formerly not possible to do for B and T cells at the very same time.Source: Camilla Engblom and Jeff MoldReference: “Spatial transcriptomics of B cell and T cell receptors exposes lymphocyte clonal dynamics” by Camilla Engblom, Kim Thrane, Qirong Lin, Alma Andersson, Hosein Toosi, Xinsong Chen, Embla Steiner, Chang Lu, Giulia Mantovani, Michael Hagemann-Jensen, Sami Saarenpää, Mattias Jangard, Julio Saez-Rodriguez, Jakob Michaëlsson, Johan Hartman, Jens Lagergren, Jeff E. Mold, Joakim Lundeberg and Jonas Frisén, 8 December 2023, Science.DOI: 10.1126/ science.adf8486The study was financed by the Swedish Research Council, the Swedish Cancer Society and the EU research and Innovation Programme Horizon 2020. Possible conflicts of interest: Camilla Engblom, Kim Thrane, Jeff Mold, Jonas Frisén, Joakim Lundeberg, and Qirong Lin are developers of a patent that covers this work. Camilla Engblom, Kim Thrane, Qirong Lin, Alma Andersson, Hosein Toosi, Sami Saarenpää, Jeff Mold, Joakim Lundeberg, and Jonas Frisén are scientific consultants for 10x Genomics, which holds copyright rights to this technology. Jeff Mold holds shares in Pacific Biosciences.
Credit: SciTechDaily.comA revolutionary method for pinpointing immune cell receptors in tissues advances illness research and treatment development.A new technique, established at Karolinska Institutet, KTH Royal Institute of Technology, and SciLifeLab, can determine special immune cell receptors and their area in tissue, a research study released in the journal Science Reports. The researchers predict that the technique will enhance the ability to determine which immune cells contribute to illness processes and open up opportunities to establish novel treatments for many diseases.Immune cells such as T and B cells are main to the bodys defense versus both infections and tumors. Credit: Johannes FrandsenResearchers at Karolinska Institutet, KTH Royal Institute of Technology, and SciLifeLab have actually now developed a method that is able to both identify the different B and T cell receptors and reveal their location in human tissue.Many Areas of Application”Since activated immune cells are typically found close to the targets that they attack, we want to be able to map the cells that are certainly closest to a tumor or infection,” states Camilla Engblom, assistant teacher at the Department of Medicine (Solna), Karolinska Institutet and one of the research studys three lead authors along with Kim Thrane, KTH/SciLifeLab, and Qirong Lin, Karolinska Institutet. It was called “Method of the Year 2020” by the journal Nature Methods.The new technique is an upgrade of the initial method that now makes it possible for researchers to map the immune cells receptors and their specific location in tissue, which was formerly not possible to do for B and T cells at the exact same time.Source: Camilla Engblom and Jeff MoldReference: “Spatial transcriptomics of B cell and T cell receptors reveals lymphocyte clonal characteristics” by Camilla Engblom, Kim Thrane, Qirong Lin, Alma Andersson, Hosein Toosi, Xinsong Chen, Embla Steiner, Chang Lu, Giulia Mantovani, Michael Hagemann-Jensen, Sami Saarenpää, Mattias Jangard, Julio Saez-Rodriguez, Jakob Michaëlsson, Johan Hartman, Jens Lagergren, Jeff E. Mold, Joakim Lundeberg and Jonas Frisén, 8 December 2023, Science.DOI: 10.1126/ science.adf8486The research study was financed by the Swedish Research Council, the Swedish Cancer Society and the EU research and Innovation Programme Horizon 2020.
