The study reveals that the RNA modification N4-acetylcytidine (ac4C) plays an essential role in the formation of tension granules and the cellular reaction to stress, providing new insights into the molecular systems involved and recognizing potential targets for disease treatment.Stress granules play an essential role in the stress action, occurring from the aggregation of non-translating mRNAs and proteins. Alterations to mRNA can change the attributes of the nucleobases, affecting crucial processes like translation, splicing, and the positioning of specific transcripts.The scientists reveal that the RNA modification N4-acetylcytidine (ac4C) on mRNA partners with records improved in stress granules and that stress granule localized transcripts with ac4C are particularly translationally regulated.They also reveal that ac4C on mRNA can mediate the localization of proteins to stress granules. While stress granule assembly and disassembly can be controlled by numerous post-translational modifications the impact of RNA modifications on their function, dispersal, and development remains largely unclear.Impact of ac4C on Stress Granules and Cellular Stress ResponseThe RNA modification N4-acetylcytidine (ac4C) has just recently been shown to be transferred on mRNA and control translation performance.