The research study team analyzed the distribution of MC4Rs in the rat brain by making use of an antibody they had developed particularly to make MC4Rs visible. They discovered that MC4Rs are present solely in primary cilia of particular groups of hypothalamic neurons.The group next investigated the length of the main cilia that had MC4Rs (MC4R+ cilia) in the brains of 9-week-old (young) rats and 6-month-old (middle-aged) rats. On the other hand, MC4R+ cilia in rats on a high-fat diet reduced at a quicker rate, while those in rats on a limited diet shortened at a slower pace.Interestingly, the group also found that MC4R+ cilia that when vanished with age were regenerated in rats raised under 2 months of dietary restriction.In the research study, the team likewise utilized hereditary technologies to make MC4R+ cilia much shorter in young rats.
The research team analyzed the circulation of MC4Rs in the rat brain by utilizing an antibody they had developed particularly to make MC4Rs noticeable. They discovered that MC4Rs are present solely in primary cilia of particular groups of hypothalamic neurons.The team next examined the length of the main cilia that had MC4Rs (MC4R+ cilia) in the brains of 9-week-old (young) rats and 6-month-old (middle-aged) rats. On the other hand, MC4R+ cilia in rats on a high-fat diet plan shortened at a faster pace, while those in rats on a limited diet reduced at a slower pace.Interestingly, the group also discovered that MC4R+ cilia that once disappeared with age were regrowed in rats raised under 2 months of dietary restriction.In the study, the group also used hereditary technologies to make MC4R+ cilia shorter in young rats. These rats revealed increased food intake and reduced metabolic process, leading to weight gain.The group also administered a hormonal agent called leptin to the brains of rats with synthetically shortened MC4R+ cilia.