Biotium, a leading provider and innovator of fluorescent cellular probes for life science research study, reveals the release of ExoBrite ™ True EV Membrane Stains for detection of extracellular vesicles (EVs). ExoBrite ™ True EV Membrane Stains are novel membrane dyes created for clear distinction of EVs from non-specific particles throughout flow detection. Fluorescence nanoparticle tracking analysis (fNTA) identified the spots to be an exceptional choice over PKH with greater (near-complete) pan-EV staining. ExoBrite ™ True EV Membrane Stains are offered in 2 colors for detection in FITC or PE channels, and can be used with antibodies for multi-parameter analysis. The spots are likewise available in a standard size of 500 labelings, or trial size of 100 labelings. To find out more about ExoBrite ™ True EV Membrane Stains, go to the item page.Flow cytometry and fNTA analysis of SEC purified MCF-7-derived EVs stained with ExoBrite ™ 515/540 True EV Membrane Stain and PKH67. Left: Analysis on a CytoFLEX LX circulation cytometer shows ExoBrite ™ True EV Membrane Stain had the ability to differentiate EVs from background particles, unlike PKH67. Stained EVs were detected in the FITC channel. Right: In ZetaView ® fNTA, ExoBrite ™ 515/540 True EV Membrane Stain showed almost complete coverage of EVs, much higher than PKH67. Total EV number was mBIotium”We are extremely thrilled about the brand-new ExoBrite ™ True EV Membrane Stains” states Dr. Alexis Madrid, head of Biotiums EV product development group. “We understand that numerous scientists want excellent, tidy pan-EV discolorations and EV membrane spots, and for many years many people have actually struggled to get great outcomes staining EVs with other membrane dyes. Now researchers at Biotium have created a set of membrane dyes that are specifically created for EVs. These dyes will permit scientists to confidently stain their EVs and achieve higher coverage and cleaner results than ever in the past.”Features of ExoBrite ™ True EV Membrane Stains: Lipophilic membrane dyes created for pan-EV labelingSuperior option to common membrane dyes like PKH, DiO, DiI, DiDNear-complete protection of EVs in a sample Broad compatibility with EVs separated from different sourcesCompatible with antibody-co-stainingAfter recognizing an unmet requirement for trusted probes for EV detection, particularly in circulation cytometry, Biotium sought to release a varied line of unique reagents aimed at addressing these obstacles, starting with the 2021 release of ExoBrite ™ CTB EV Staining Kits (formerly ExoBrite ™ EV Membrane Staining Kits). 2022 saw the intro of the ExoBrite ™ Antibody Conjugates (CD81, cd63, and cd9) for circulation cytometry and western blotting, followed by ExoBrite ™ WGA (wheat bacterium agglutinin conjugates) and Annexin V EV Surface Stains. An ExoBrite ™ EV Surface Stain Sampler Kit that includes samples of ExoBrite ™ CTB, WGA, and Annexin V spots is likewise now available, as well as ExoBrite ™ Streptavidin Magnetic Beads that can be integrated with biotinylated antibodies for effective EV capture and seclusion. Specific and delicate reagents for identifying EVs for detection by circulation cytometry and other applications continues to be a strong pursuit for Biotium, and ExoBrite ™ True EV Membrane Stains even more includes to their flexible repertoire of robust reagents for EV research study.
To find out more about ExoBrite ™ True EV Membrane Stains, visit the product page.Flow cytometry and fNTA analysis of SEC purified MCF-7-derived EVs stained with ExoBrite ™ 515/540 True EV Membrane Stain and PKH67. Total EV number was mBIotium”We are really thrilled about the new ExoBrite ™ True EV Membrane Stains” says Dr. Alexis Madrid, head of Biotiums EV item advancement group. “We understand that lots of researchers want excellent, tidy pan-EV spots and EV membrane stains, and for years numerous people have struggled to get excellent outcomes staining EVs with other membrane dyes.
