In 2001, researchers finished the very first draft of the human genome utilizing Sanger DNA sequencing, a pricey and laborious approach when sequencing big quantities of DNA. Slower sequencing runs can increase precision, while faster sequencing runs may reduce accuracy.1,2Ion-TorrentIon-Torrent innovation utilizes emulsion PCR to enhance adapter-ligated DNA series on the surface area of beads that are dispersed into microwells. Ion-Torrent is likewise dependent on emulsion PCR, which, like any PCR method, can introduce amplification predispositions since specific series are simpler to enhance than others.1,3Single-molecule real-time (SMRT) sequencingIn SMRT sequencing, researchers cap DNA molecules with single-stranded barrette adapters, and sequencing takes place without multiple amplification actions. The longer DNA sequences can likewise cover duplicating DNA, which permits scientists to cover more of the genome.1,4 High Throughput Sequencing ApplicationsWhole genome sequencingWith their large size and various repeat sequences, whole genomes continue to present the biggest sequencing obstacles. With no need for a template, scientists use SMRT sequencing to put together unique genomes from understudied species.5 Exome sequencingExome sequencing is the fastest and most cost-effective alternative detection technique, and it offers a fast method to discover heritable mutations in a scientific setting.
In 2001, researchers finished the first draft of the human genome utilizing Sanger DNA sequencing, a expensive and tiresome technique when sequencing big amounts of DNA. Slower sequencing runs can increase accuracy, while faster sequencing runs might reduce accuracy.1,2Ion-TorrentIon-Torrent technology uses emulsion PCR to magnify adapter-ligated DNA sequences on the surface of beads that are distributed into microwells. The longer DNA sequences can also span duplicating DNA, which enables researchers to cover more of the genome.1,4 High Throughput Sequencing ApplicationsWhole genome sequencingWith their big size and numerous repeat sequences, whole genomes continue to provide the biggest sequencing challenges.