May 18, 2024

In the Shadow of Rome: Ancient DNA Recasts Balkan History

To explore the population history of the Balkans and take a look at the impact of the increase and fall of the Roman empire, the scientists extracted DNA from 136 ancient people excavated from 20 various sites across the Balkans– defined as the area bounded by the Adriatic, Central Mediterranean, and Aegean Seas and the Middle and Lower Danube and Sava Rivers. The team focused on 3 durations: during the expansion and height of the Roman Empire (1– 250 CE), during the late Imperial period (circa 250– 550 CE), and following the Western Empires collapse (550– 1000 CE).
The researchers were surprised to discover no proof of Italian Iron Age ancestry in the Balkan populations during the height of the Roman Empire. They also discovered evidence of specific migrations into the Balkans from both within and outside the Roman Empire. From 600 CE, soon after the fall of the Western Roman Empire, there was a major increase of people from Eastern Europe.

Most ancient DNA studies focus on pre-history– before the written record– but ancient DNA methods can likewise offer insight into more current historic periods, especially when utilized in combination with historic and archeological information.
Ancient DNA: A Window into History
” Ancient DNA can offer a lot of insight into historical periods, particularly for areas where historic sources are limited or when we do not understand whether sources are biased or not,” states first author and population geneticist Iñigo Olalde of the University of the Basque Country (UPV/EHU). “For example, many historic sources from the Balkans are composed from the side of the Romans since the Slavic individuals didnt compose at that time.”
This picture shows the Mausoleum of Viminacium. Credit: Carles Lalueza-Foz
Checking out the Balkans Demographic History
Previous studies have investigated the ancestry of people who lived in Italy and England throughout and after the fall of the Roman Empire, however little is learnt about demography and origins of the Balkans throughout this time. “This region was among the far-off frontiers of the Roman Empire, which makes it fascinating to study since this is plainly a place where you would expect individuals to come in contact with people from outside the Empire, so you can evaluate things such as globalization,” says Olalde.
To explore the population history of the Balkans and examine the impact of the fluctuate of the Roman empire, the scientists extracted DNA from 136 ancient individuals excavated from 20 various websites across the Balkans– specified as the area bounded by the Adriatic, Central Mediterranean, and Aegean Seas and the Middle and Lower Danube and Sava Rivers. These websites included big Roman cities, military fortresses, and small rural towns. The team concentrated on 3 durations: throughout the growth and height of the Roman Empire (1– 250 CE), during the late Imperial period (circa 250– 550 CE), and following the Western Empires collapse (550– 1000 CE).
Method and Surprising Discoveries
To supply cultural and historical context for the hereditary information, the group worked together with regional archeologists and historians. For each tomb, they documented burial type, in addition to any things buried alongside the individuals, such as coins, precious jewelry, pottery, tools, and weapons. The researchers also utilized radiocarbon dating to confirm the age of 38 of the ancient individuals, which generated isotopic data that provide a window into those individuals diet plans.
This photograph shows a Roman aqueduct that provided water to Viminacium, a big Roman city. Credit: Carles Lalueza-Foz
The researchers were shocked to find no proof of Italian Iron Age origins in the Balkan populations during the height of the Roman Empire. They likewise discovered evidence of specific migrations into the Balkans from both within and outside the Roman Empire.
” This was the only complete Eastern African person that we examined, and he was also a clear outlier with regard to the diet compared to the remainder of the individuals buried in the very same necropolis, which tells us that this private plainly grew up outside the borders of the Roman Empire,” states Lalueza-Fox.
Late Imperial Period and Slavic Influence
During the late Imperial duration, in between 250 and 550 CE, the researchers detected migrants with blended origins from Northern Europe and the Pontic-Kazakh steppe. “We found that those 2 ancestries– central/northern European and Sarmatian-Scythian– tended to come together, which suggests that these are most likely to have been multi-ethnic confederations of moving individuals,” states senior author and population geneticist David Reich of Harvard University.
From 600 CE, quickly after the fall of the Western Roman Empire, there was a significant influx of individuals from Eastern Europe. These migrations coincide with recorded Slavic migrations, however the DNA analysis supplies insight into the scale of these migrations that is impossible to obtain from historic resources.
Future Directions and Study Enhancements
” There have actually been debates about how impactful these migrations were and to what degree the spread of Slavic language was largely through cultural influences or motions of individuals, however our study reveals that these migrations had a profound market impact,” states Reich. “More than half of the origins of the majority of peoples in the Balkans today comes from the Slavic migrations, with around a third Slavic origins even in countries like Greece where no Slavic languages are spoken today.”
The group are already preparing what they call “variation two” of the research study, which will benefit from enhancements in ancient DNA innovations. “We are now able to sequence hundreds of people from the very same website, so we can go to another level of resolution and start to comprehend more about the social interactions and kinship in between the different people,” states Olalde.
Reference: “A hereditary history of the Balkans from Roman frontier to Slavic migrations” by Iñigo Olalde, Pablo Carrión, Ilija Mikić, Nadin Rohland, Swapan Mallick, Iosif Lazaridis, Matthew Mah, Miomir Korać, Snežana Golubović, Sofija Petković, Nataša Miladinović-Radmilović, Dragana Vulović, Timka Alihodžić, Abigail Ash, Miriam Baeta, Juraj Bartík, Željka Bedić, Maja Bilić, Clive Bonsall, Maja Bunčić, Domagoj Bužanić, Mario Carić, Lea Čataj, Mirna Cvetko, Ivan Drnić, Anita Dugonjić, Ana Đukić, Ksenija Đukić, Zdeněk Farkaš, Pavol Jelínek, Marija Jovanovic, Iva Kaić, Hrvoje Kalafatić, Marijana Krmpotić, Siniša Krznar, Tino Leleković, Marian M. de Pancorbo, Vinka Matijević, Branka Milošević Zakić, Anna J. Osterholtz, Julianne M. Paige, Dinko Tresić Pavičić, Zrinka Premužić, Petra Rajić Šikanjić, Anita Rapan Papeša, Lujana Paraman, Mirjana Sanader, Ivana Radovanović, Mirjana Roksandic, Alena Šefčáková, Sofia Stefanović, Maria Teschler-Nicola, Domagoj Tončinić, Brina Zagorc, Kim Callan, Francesca Candilio, Olivia Cheronet, Daniel Fernandes, Aisling Kearns, Ann Marie Lawson, Kirsten Mandl, Anna Wagner, Fatma Zalzala, Anna Zettl, Željko Tomanović, Dušan Keckarević, Mario Novak, Kyle Harper, Michael McCormick, Ron Pinhasi, Miodrag Grbić, Carles Lalueza-Fox and David Reich, 7 December 2023, Cell.DOI: 10.1016/ j.cell.2023.10.018.
This research was supported by the Spanish Ministry of Science of Innovation, la Caixa” Foundation, the Natural Sciences and Engineering Research Council of Canada, the Ministry of Science and Education of the Republic of Croatia, the National Institutes of Health, the John Templeton Foundation, the Allen Discovery Center, the Paul G. Allen Family Foundation, and the Howard Hughes Medical Institute.

A thorough DNA analysis of the Balkan population from 1 to 1000 CE challenges the assumed Roman influence, revealing no Italian genetic qualities. Rather, the study uncovers considerable ancestry from Western Anatolia, Europe, and the Pontic-Kazakh Steppe, with a significant Slavic migration shaping modern-day Balkan genes. Credit: SciTechDaily.com
An innovative study reverses previous assumptions about Roman influence in the Balkans, showing a diverse genetic heritage mainly influenced by Slavic migrations, instead of Italian origins.
Despite the Roman Empires extensive military and cultural impact on the neighboring Balkan peninsula, a DNA analysis of individuals who lived in the region in between 1 and 1000 CE found no hereditary proof of Iron Age Italian origins. Rather, a study published December 7 in the journal Cell revealed successive waves of migrations from Western Anatolia, central and northern Europe, and the Pontic-Kazakh Steppe throughout the Empires reign.
The Impact of Slavic Migration
From the 7th century CE onwards (coincident with the fall of the Western Roman Empire), great deals of people emigrated from Eastern Europe, most likely related to the arrival of Slavic-speaking populations, which resulted in contemporary Balkan homeowners having 30%– 60% Slavic ancestry seen in present-day Balkan individuals.
Skull of an individual of East African ancestral origin found in Viminacium, with the oil lamp including an eagle discovered in his tomb. Credit: Miodrag (Mike) Grbic
” We discovered this hereditary signal of Slavic migration all throughout the Balkans,” states senior author and paleogenomicist Carles Lalueza-Fox of the Institute of Evolutionary Biology (IBE: CSIC-Universitat Pompeu Fabra) and Museu de Ciències Naturals de Barcelona. “This could have crucial social and political implications considered that the Balkans has had a long history of dispute associated with their perceived identities.”