April 29, 2024

Improving Reproducibility with Automated Liquid Handling

Transferring and dispensing liquids are mundane and easy jobs that have profound impacts on experimental outcomes. Using manual single-channel pipettes to move samples or reagents is commonplace among scientists. Integrating multi-channel pipettes enhances efficiency to a degree, however that advantage reduces as the number and scale of experiments increase. Researchers can improve the speed, accuracy, and reproducibility of their liquid handling by making the switch to an automated system.While by hand giving liquids, user-to-user variation in pipette managing introduces errors. Improper pipette handling, disparity in goal and dispensing rhythm, and irregularity in pipetting speed prevail examples of user error that lower experimental efficiency and precision. Excessive or rough pipetting of protein or nucleic acid solutions deteriorates sample quality. Additionally, users can quickly cross-contaminate their samples, which causes undependable results or stopped working experiments.In addition to operator mistake, the attributes of different liquid types contribute to errors. Viscous liquids do not stream quickly, and as viscosity increases, so do pipetting errors. Viscous liquids have a sluggish flow rate, and volume loss is unavoidable if they are not given slowly. Utilizing an unsuitable pipette for the liquid type changes the dispensing volume.Precisely pipetting small volumes demands careful dealing with skills. With increasing complexity and throughput in experiments that require specifically small volumes, such as qPCR, drug library screening, genomics, and molecular diagnostics, manual pipetting substances human mistake, considerably lowering precision.Automating liquid transfers resolves the problems related to manual pipetting. Enhanced reproducibility and accuracy are the most apparent advantages, as automation eliminates user irregularity. In addition, automation considerably enhances throughput, optimizes efficiency, and obliterates user mistake, making it an appealing choice for low- and high-throughput laboratory tasks.The Eppendorf epMotion ® series automated liquid handling workstations uses a service to increase accuracy, flexibility, and scalability in experiments performed in both small research groups and huge laboratories. Each epMotion system includes autoclavable single- or multi-channel dispensing tools for volumes from 200 nL to 1,000 μL, and an optical sensor that quality checks the deck positions before each run and identifies the liquid type and volumes in vessels in a contact-free manner. In addition, the sensing unit distinguishes the number and type of packing ideas prior to the start of an operation.Each model in the epMotion series can be customized for particular applications. For example, epMotion 96 is a simple 96-well multi-channel pipette with parallel piston motions that guarantee precise and complete reagent distribution in 96- and 384-well plates. epMotion 5070 is a compact service for regular applications, while epMotion 5073 systems are tailored for PCR setup, nucleic acid purification, and next generation sequencing. Finally, the biggest epMotion 5075 is a diverse tool for applications including real-time PCR set-up, next generation sequencing, and cell-based assays. The epMotion 5070, 5073, and 5075 systems have multiple deck positions that assist in a series of tasks, from regular serial dilutions to intricate operations. Additionally, these instruments reliably give the low volumes required of high-throughput assays.Finally, Eppendorf epMotion liquid-handling systems are geared up with intuitive and user-friendly epBlueTM software application. The software includes a big repository of labware and liquid types to improve pipetting for basic applications, such as pipetting in 96- or 384-well plates. With the epMotion systems and accessory dispensing tools, researchers execute easy explores precision and boost reproducibility in complex, high-throughput experiments, such as drug screening, genomics, proteomics, and cell-based assays..

Scientists can improve the speed, precision, and reproducibility of their liquid handling by making the switch to an automatic system.While manually giving liquids, user-to-user variation in pipette handling introduces errors. With increasing complexity and throughput in experiments that need particularly small volumes, such as qPCR, drug library screening, genomics, and molecular diagnostics, manual pipetting substances human mistake, drastically minimizing precision.Automating liquid transfers resolves the problems associated with manual pipetting. Each epMotion system consists of autoclavable single- or multi-channel dispensing tools for volumes from 200 nL to 1,000 μL, and an optical sensing unit that quality checks the deck positions prior to each run and spots the liquid type and volumes in vessels in a contact-free manner. The software application includes a large repository of labware and liquid types to simplify pipetting for standard applications, such as pipetting in 96- or 384-well plates.