Visualization of local damage in skeletal muscle fibers (soleous and gastro) of young mice after exercise, by staining with local damage markers such as Filamina C (green) and Evans Blue (red), and nuclei (in blue) of fibers. Credit: UPF/ CNIC.
A new research study led by Spanish researchers describes a brand-new system for muscle repair work after physiological damage depending on the rearrangement of muscle fiber nuclei, and separately of muscle stem cells.
Muscle is understood to regrow through a complex procedure that relies and includes numerous actions on stem cells. Now, a brand-new research study led by researchers at Pompeu Fabra University (UPF, Spain)/ Centro Nacional de Investigationes Cardiovasculares (CNIC, Spain)/ CIBERNED (Spain) and Instituto de Medicina Molecular João Lobo Antunes (iMM, Portugal), released on 15 October in the journal Science, explains a brand-new mechanism for muscle repair work after physiological damage counting on the rearrangement of muscle fiber nuclei, and separately of muscle stem cells. This protective system paves the way to a more comprehensive understanding of muscle repair work in physiology and disease.
In spite of the plasticity of these fibers, their contraction can be associated with muscle damage. Muscle regrowth has actually been examined in depth in recent decades, a lot of research studies centered on mechanisms including several cells, including muscle stem cells, which are needed when extensive muscle damage occurs.”.
” In this research study we discovered an alternative mechanism of muscle tissue repair that is muscle-fiber autonomous,” says Pura Muñoz-Cánoves, ICREA teacher and primary detective at UPF and the CNIC, and research study leader. Scientists (including Antonio Serrano (UPF) and Mari Carmen Gómez-Cabrera (University of Valencia and INCLIVA) utilized various in vitro designs of injury and models of exercise in human beings and mice to observe that upon injury, nuclei are drawn in to the damage website, speeding up the repair work of the contractile systems. Next, the group dissected the molecular system of this observation: “Our explores muscle cells in the lab revealed that the motion of nuclei to injury websites led to the local shipment of mRNA molecules. These mRNA molecules are translated into proteins at the site of injury to serve as structure blocks for muscle repair work,” explains William Roman. “This muscle fibre self-repair process occurs rapidly both in mice and in human beings after exercise-induced muscle injury, and thus represents a time- and energy-efficient protective system for the repair work of minor lesions,” adds Pura Muñoz-Cánoves.
In addition to its ramifications for muscle research, this research study also presents more general principles for cell biology, such as the movement of nuclei to injury sites. “One of the most interesting things about these cells is the movement during the advancement of their nuclei, the biggest organelles inside the cell, but the reasons nuclei move are mainly unknown. Now, we have actually revealed a practical significance for this phenomenon in their adult years during cell repair work and regeneration,” says Edgar R. Gomes, group leader at the Instituto de Medicina Molecular and a teacher at the Faculty of Medicine at the University of Lisbon, who co-led the study.
On the value of these discoveries, Pura Muñoz-Cánoves, Antonio Serrano and Mari Carmen Gómez-Cabrera concur that: “This finding makes up a crucial advance in the understanding of muscle biology, in physiology (consisting of exercise physiology) and muscle dysfunction.”.
Referral: “Muscle repair work after physiological damage depends on nuclear migration for cellular restoration” by William Roman, Helena Pinheiro Mafalda R. Pimentel, Jessica Segalés, Luis M. Oliveira, Esther García-Domínguez, Mari Carmen Gómez-Cabrera, Antonio L. Serrano, Edgar R. Gomes and Pura Muñoz-Cánoves, 15 October 2021, Science.DOI: 10.1126/ science.abe5620.
The work was performed at UPF/CNIC/CIBERNED and at the iMM in collaboration with the University of Valencia/INCLIVA.
This study was funded by the European Research Council, Association Française contre les Myopathies, the European Molecular Biology Organization, the Human Frontiers Science Program, and the Spanish Ministry of Science.
Pompeu Fabra University (Spain).
UPF (and the CEXS Department in particular) and CNIC are two centers of quality (María de Maeztu and Severo Ochoa, respectively) devoted to Biomedicine and Cardiovascular research study.
The Network Center for Biomedical Research in Neurodegenerative Diseases (CIBERNED) is a research study body founded by the Spanish Government to fuse scientific and standard research study targeting pathologies of fantastic value for the National Health System.
Instituto de Medicina Molecular (iMM; Portugal).
The iMM– Instituto de Medicina Molecular João Lobo Antunes is a leading Portuguese private non-profit research study institute that uses a lively clinical environment, aiming to support innovative concepts in standard, medical and translational biomedical research.
About the CNIC.
The Centro Nacional de Investigaciones Cardiovasculares (CNIC), directed by Dr. Valentín Fuster, is devoted to cardiovascular research and the translation of understanding acquired into real benefits for patients. The CNIC, acknowledged by the Spanish government as a Severo Ochoa center of excellence, is financed through a pioneering public-private partnership in between the federal government (through the Carlos III Institute of Health) and the Pro-CNIC Foundation, which unites 12 of the most crucial Spanish private business.
“This muscle fibre self-repair process takes place quickly both in mice and in people after exercise-induced muscle injury, and thus represents a time- and energy-efficient protective mechanism for the repair work of small lesions,” adds Pura Muñoz-Cánoves.
Now, a brand-new research study led by scientists at Pompeu Fabra University (UPF, Spain)/ Centro Nacional de Investigationes Cardiovasculares (CNIC, Spain)/ CIBERNED (Spain) and Instituto de Medicina Molecular João Lobo Antunes (iMM, Portugal), released on 15 October in the journal Science, explains a brand-new system for muscle repair after physiological damage relying on the rearrangement of muscle fiber nuclei, and separately of muscle stem cells. Skeletal muscle tissue, the organ responsible for mobility, is formed by cells (fibers) that have more than one nucleus, an almost special function in our body. Despite the plasticity of these fibers, their contraction can be associated with muscle damage. Muscle regrowth has been investigated in depth in recent decades, most studies centered on mechanisms including numerous cells, consisting of muscle stem cells, which are needed when extensive muscle damage occurs.”.