December 5, 2023

Infographic: Two Guide RNAs Make for Large, Stable Insertions

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Two prime modifying guide RNAs (pegRNAs), deliver Cas9 enzymes to targeted areas of the genome. The enzymes nick opposite hairs of the DNA at various points, at which point the pegRNA functions as a template for the preferred gene insertion. This results in two single-stranded DNAs (ssDNA) that bind to each other due to their partially complementary sequence and, if they outcompete the initial DNA, are integrated into the genome and completed by the host cells existing DNA repair machinery. Due to the fact that two pegRNAs are used, GRAND modifying can place sectors of approximately 1,000 base pairs long– more than what one pegRNA might provide alone.THE SCIENTIST STAFFRead the complete story.