The lower temperature in RPA increases the danger of nonspecific amplification, so Merkx sought a sensitive solution.”The appeal of bioluminescence is its simplicity,” said Merkx. The group split luciferase, an enzyme that produces bioluminescence, in between two dCas9 proteins. The team tested this platform on COVID-19 samples and spotted SARS-CoV-2 RNA within 30 minutes.
The lower temperature level in RPA increases the risk of nonspecific amplification, so Merkx sought a delicate solution.”The beauty of bioluminescence is its simpleness,” stated Merkx. The group tested this platform on COVID-19 samples and spotted SARS-CoV-2 RNA within 30 minutes.
