Recent research highlights how the influenza virus might penetrate the brain, causing severe influenza-associated encephalopathy (IAE). This study suggests that the virus targets endothelial cells forming the brain’s protective barrier, and that antivirals inhibiting viral protein processes could be effective treatments. Credit: SciTechDaily.com
Some influenza-associated brain disorders may be caused by the virus entering the brain and making proteins, suggesting that antivirals that block protein production might be an effective treatment, according to new research by Osaka University.
It might start simply, with a cough or sneeze…but in some cases, the flu virus can make its way to your brain, causing serious symptoms or even death through a disease known as influenza-associated encephalopathy (IAE).
Now, in a study published in Acta Neuropathologica, researchers have revealed that IAE may be caused by the virus entering the brain through a specific cell type, and have identified possible treatment strategies.
Although IAE is increasingly common, surprisingly little is known about how the influenza virus actually gets into the brain and causes symptoms of encephalopathy (a general term for brain disorders). Notably, a definitive treatment for IAE remains lacking, something that researchers from Osaka University wanted to address.
To study how influenza viruses might cause IAE, the research team used a range of methods. As well as looking for virus particles in the brains of people who died from IAE, they created a mouse model of the disease by injecting influenza A virus into the blood. They also used cell culture methods to look at how well the virus infects different cell types.
“In the human brains, the virus-injected mice, and the cultured cells, the influenza virus tended to accumulate in endothelial cells,” explains lead author of the study Shihoko Kimura-Ohba. “These cells create a barrier between the blood and the brain, and are important for protecting the brain from harmful substances.”
The IAE pathogenesis, with severe brain edema caused by IAV, could be established without viral proliferation: Influenza virus protein was produced and accumulated in the influenza virus-infected endothelial cells (EC). Without producing daughter virus, accumulated viral protein induced necrosis of EC and disruption of the blood-brain barrier leading to vascular leakage and bleeding. Credit: ©2024 Kimura-Ohba
Accordingly, in both the human brains and the mouse model, the barrier between the blood and the brain was compromised. Furthermore, the researchers noticed that the virus wasn’t actually reproducing within these endothelial cells—but there were a lot of proteins made by the virus.
“When we saw this viral protein accumulation in the brain, we realized that antiviral medications aimed at stopping viral proliferation were unlikely to help,” says Tomonori Kimura, senior author of the study. “However, antivirals aimed at stopping viral transcription and translation—vital processes for making proteins—are also available.”
When the team tried these classes of antivirals in endothelial cells treated with influenza virus, they observed less viral proteins and cell death. In the mouse model of IAE, these antivirals were also very effective when used early enough, suggesting that they may be useful in human patients.
Given that many different kinds of viruses, including COVID-19, can cause encephalopathy, these findings have wide-ranging impacts. Moreover, although IAE care is improving, more than half of all patients die or have long-lasting symptoms. A better understanding of how viruses can cause encephalopathy will be important for developing new, effective treatments.
Reference: “Viral entry and translation in brain endothelia provoke influenza-associated encephalopathy” by Shihoko Kimura-Ohba, Mieko Kitamura, Yusuke Tsukamoto, Shigetoyo Kogaki, Shinsuke Sakai, Hiroaki Fushimi, Keiko Matsuoka, Makoto Takeuchi, Kyoko Itoh, Keiji Ueda and Tomonori Kimura, 30 April 2024, Acta Neuropathologica.
DOI: 10.1007/s00401-024-02723-z